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KMID : 1156220140400030255
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Journal of Environmental Health Sciences
2014 Volume.40 No. 3 p.255 ~ p.263
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Fungicidal Efficacy of a Fumigation Disinfectant with Ortho-phenylphenol as an Active Ingredient against Trichophyton mentagrophytes, Candida albicans and Aspergillus niger
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Park Eun-Kee
Lee Soo-Ung
Cho Ki-Yung
Kim Yong-Pal
Yoo Chang-Yeul
Kim Suk
Lee Hu-Jang
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Abstract
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Objectives: This study evaluated the fungicidal efficacy of a fumigant containing 20% ortho-phenylphenol against Trichophytone mentagrophytes (T. mentagrophytes), Candida albicans (C. albicans) and Aspergillus niger (A. niger).
Methods: Five replicates of each carrier were contaminated by depositing 0.05 mL of each fungal suspension. After drying, two carriers without exposure to the fumigant and three carriers with exposure to the fumigant were left in a sealed room (25 m3) at 21±0.5oC and 60±10% relative humidity for 15 hours. Immediately after removal from the test room, each carrier was transferred into recovery diluent and suspended, diluted and inoculated. After incubation, the numbers of each colony were counted, and the parameter values (N, T, d) were calculated.
Results: The working culture suspension number (N value) of T. mentagrophytes, C. albicans and A. niger were 1.0×108, 1.2×108 and 5.7×107 CFU/mL, respectively. All the colony numbers on the carriers exposed to the fumigant (n1, n2, n3) were higher than 0.5N1 (the number of fungal test suspensions by pour plate method), 0.5N2 (the number of fungal test suspensions by filter membrane method) and 0.5N1, respectively. In addition, all mean numbers of test strains recovered on the control-carriers (T value) were over 106 CFU/mL. For the fungicidal effect of the fumigant, all numbers of fungal reductions after exposure of the fumigant (d value) were 4 logCFU/mL.
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KEYWORD
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Aspergillus niger, Candida albicans, Fumigation disinfectant, Ortho-phenylphenol, Trichophyton mentagrophytes
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